Enumeration of Non-culturable bacteria using CTC ???????
Douglas B. Kell
dbk at aber.ac.uk
Mon Jul 24 03:32:56 EST 1995
In article <3utjht$m6m at columba.udac.uu.se> Veljo Kisand <veljo.kisand at limno.uu.se> writes:
>cs9040 at wlv.ac.uk (Chris Thomas) wrote:
>>Does anybody happen to know where I can buy some 5-cyano-2,3-ditolyl
>>tetrazolium chloride (CTC) from? I have tried the usual suppliers
>>e.g Sigma, Fisons etc, with no joy.
>>If anybody using the dye could point me in the right direction I would
fax: 00-49 62 21 76 46 20
5-cyano-2,3-ditolyl tetrazolium chloride (CTC), Catalogue Number 19292
>I really don't know where You can buy but maybe You can a little share
>knowledge about for what and how You like to use CTC. Just the subject of
>your posting was interestin for me, I working also with non-culturable
>bacteria (freshwater lake bacteria).
Kaprelyants, A.S. & Kell, D.B. (1993) The use of 5-cyano-2,3-ditolyl tetrazolium chloride
and flow cytometry for the visualisation of respiratory activity in individual cells of
Micrococcus luteus. J. Microbiol. Methods. 17, 115-122.
Kaprelyants, A.S., Gottschal, J.C. & Kell, D.B. (1993) Dormancy in nonsporulating bacteria.
FEMS Microbiol. Rev. 104, 271-286.
Kaprelyants, A.S. & Kell, D.B. (1993) Dormancy in stationary-phase cultures of
Micrococcus luteus: flow cytometric analysis of starvation and resuscitation. Appl. Env.
Microbiol. 59, 3187-3196.
Votyakova, T.V., Kaprelyants, A.S. & Kell, D.B. (1994) Influence of viable cells on the
resuscitation of dormant cells in Micrococcus luteus cultures held in extended stationary
phase. The population effect. Appl. Env. Microbiol. 60, 3284-3291.
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