Growth of bacteria in controlled atmosphere

R.S. Perry rsperry at
Wed Jul 26 09:05:42 EST 1995

I am attempting to grow isolate Gallionella ferruginea. The procedure is
lengthy requiring many serial dilutions.  The situation is further
complicated by the fact that growth tubes must be kept in an atmosphere of
94%N2, 5%CO2 and 1%O2.  I can think of ways of achieving this but does anyone
have any practical experience of achieving such.  If so I would be very
grateful if they could e-mail me informing me of their method.  I do have
access to a Gallenkamp CO2 incubator but this only seems to allow CO2
	Thanks in advance
		Robin (rsperry at

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