Role of deoR and plasmids
aj088 at FreeNet.Carleton.CA
Wed Jul 26 18:40:24 EST 1995
benedik at uh.edu (benedik at uh.edu) writes:
> I was reading in the Gibco/BRL catalog that strains carrying the deoR
> mutation allows improved uptake of large plasmids.
> Does anyone know the basis for this? Is it improved transformation by
> large plasmids? Improved replication? Would this have an impact on
> production of large amounts of plasmid DNA even from smaller plasmids?
deoR is (as i recall) is a negative regulator for the synthesis
of deoxyribonucleotides. By knocking it out the cell overproduces
dNTPs which favors the continued replication of plasmids,
paticularly if the are large..
More information about the Microbio