Role of deoR and plasmids

Lynn Bry aj088 at FreeNet.Carleton.CA
Wed Jul 26 18:40:24 EST 1995


benedik at uh.edu (benedik at uh.edu) writes:
> I was reading in the Gibco/BRL catalog that strains carrying the deoR
> mutation allows improved uptake of large plasmids.
> 
> Does anyone know the basis for this? Is it improved transformation by
> large plasmids? Improved replication? Would this have an impact on
> production of large amounts of plasmid DNA even from smaller plasmids?
> 
> 
deoR is (as i recall) is a negative regulator for the synthesis
of deoxyribonucleotides. By knocking it out the cell overproduces
dNTPs which favors the continued replication of plasmids,
paticularly if the are large..

ln




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