HELP!! Searching for Transcription Factors
buddyw at umich.edu
Fri Jun 9 15:37:53 EST 1995
I am studying the regulation of a metabolic gene in E.coli. Our
laboratory has identified a transcription factor that directly interacts
with the 5' upstream and promoter regions of our metabolic gene.
Further, I have mapped the binding sites of the factor by DNase I
footprinting. The binding sites are a couple hundred bases pairs
upstream of the start site of transcription, suggesting the presence of
Thus, what I would like to be able to do is search some database of
known recognition sequences of transcription factors that bind to the
promoter of my gene. (I would like to submit my long upstream and
promoter sequence, maybe 2-300bp's, and have the computer churn out a
list of factors that might bind.) I have found two databases in Europe
that can do something like this, but both are for Eukaryotes!!! Arghh!
Please E-mail me (buddyw at umich.edu) if you can help/have suggestions.
Thanks in advance...
More information about the Microbio