Subcloning questions

David J. Kanter 75444.2004 at CompuServe.COM
Thu Mar 2 10:35:05 EST 1995


Got some questions on a DNA subclone/restriction map lab I did.
1. In one well of my gel separation, I saw a bright haze in the 
bottom lane rather than a band. Is the sample degrading as a goes 
across the gel? What’s going on? (Used ethidium bromide with E. 
coli vector)
2. Why ultracentrifuge to purify DNA?
3. Why would a restriction enzyme NOT cut a plasmid? Because a 
protein in gel bound to restriction site? Why partial digests?
Thanks in advance, Dave



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