(Fwd) smearing on pulse field gel

MS WF HONG [GEN]53872 GEN282E at ccs1.cc.monash.edu.au
Tue Mar 7 02:42:29 EST 1995

Subject:       smearing on pulse field gel

Hi netters,

     I am working on genomic mapping of Pseudomanas by pulse field gel 
electrophoresis. One particular strain of a species I'm working with 
produces a smear when digested within agarose plugs whereas other 
strains of the same species can be cut successfully by the same rare 
cutting enzymes such as SwaI or CeuI. However, they all can be cut by 
SpeI. Does anyone have any suggestions? Any help at all will be greatly 

Many thanks in advance.

WF Hong

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