Need help on growing Clostridium & Lactococcus

MikeC12053 mikec12053 at
Sat Mar 11 10:23:04 EST 1995

>I am trying to grow Clostridium sporogenes & Lactococcus lactis
>for an Introductory Microbiology Lab course.  The Clostridium
>I've ordered came in Thioglycollate broth and the Lactococcus 
>came in some kind of Tomato juice yeast extract broth.  However
>I would like to grow them on solid media, slant or plate, not
>broth.  I have tried both culture on nutrient, tryptic soy, and
>brain heart infusion agar, MacConkey, blood, and chocolate agar
>with no luck.  Would anyone out there wiht any kind of experience
>working with these two organisms please me some insight.  Please
>be as specific as possible as to what solid media to use and 
>under what condition to maintain the culture.  Thank you kindly.

Try growing Clostridia on Liver Veal Agar (LVA) and Lactobacilli on DeMan
Rogosa Sharpe (MRS) agar. Both are available from Difco and other media
suppliers. Clostridia must be incubated anaerobically. Lactobacilli
sometimes grow better anaerobically.


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