CELL #

John W. Patching John.Patching at UCG.IE
Wed Mar 29 04:14:45 EST 1995


>Resent-date: Tue, 28 Mar 1995 22:37:53 +0000 (UT)
>Date: Tue, 28 Mar 1995 20:24:07 +0000 (GMT)
>Resent-from: server-daemon at dl.ac.uk
>From: wsargent at badlands.NoDak.edu (Wayne A Sargent)
>Subject: CELL #

>
>I would truly appreciate a formula for converting an OD reading
>into an approximate # of bacterial cells/ml.
>
>THANX in ADVANCE!
>
Sorry, Wayne - there is no "quick fix"!  Get  your culture/cell suspension
- do a cell count - do dilutions - prepare a standard curve.  Finding
"magic" formulae and deciding which is the "right" one to use will probably
take longer!
 Donning my editor's/referee's hat, I feel that quoting unconverted OD's or
a conversion done using someone's "universal" conversion factor is not far
removed from saying that your culture looks "cloudy" or "not so cloudy".
OD's are so easy to take.  Please folks - do that little bit extra and
convert them into something which is meaningful and reproducable by other
scientists.

John W. Patching
The Martin Ryan Marine Science Institute
University College Galway, Ireland

Email: John.Patching at UCG.IE
Phone: +353-91-24411 Ext 2398
Fax:       +353-91-750456





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