CELL #

Scott Kellogg kellogg at raven.csrv.uidaho.edu
Wed Mar 29 11:31:33 EST 1995


Try the following:

OD550 = (1.0315e-8)(cell number/ml)+1.7633e-3

The r-squared for this regression is 0.9997 and the data are for the 
genus Vibrio (taken from Primrose & Wardlaw, 1982, Sourcebook of 
experiments for the teaching of microbiology, p. 151).  This equation is 
valid only for ODs from 0.02 to 0.50, and in general one should be use 
ODs less than 1 for accuracy (so dilute).


Wayne A Sargent (wsargent at badlands.NoDak.edu) wrote:
: I would truly appreciate a formula for converting an OD reading 
: into an approximate # of bacterial cells/ml.

: THANX in ADVANCE!

: ------------------------------------------------------------------------------

: Wayne Sargent
: Department of Plant Sciences
: Loftsgard Hall, Room 166	    phone : 701-231-8057
: North Dakota Sate University	    fax   : 701-231-8474
: Fargo, ND          58105	    e-mail: wsargent at badlands.NoDak.edu

: ------------------------------------------------------------------------------


--
===================================================================
Scott T. Kellogg, Dept. of Microbiol., Mol. Biol. & Biochem.
Univ. of Idaho, Moscow, ID 83844-3052  Internet: kellogg at uidaho.edu
Phone: 208-885-6966      Fax: 208-885-6518



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