K N and P J Harris
ecoli at cix.compulink.co.uk
Sat Nov 4 05:14:54 EST 1995
> bionet/microbiology #944, from brucerat at aol.com, 961 chars, 20 Oct
1995 09:21:50 -0
> Article: 1895 of bionet.microbiology
> From: brucerat at aol.com (BruceRat)
> Newsgroups: bionet.microbiology
> Subject: Amylase--Michealis-Menten kinetics?
> Date: 20 Oct 1995 09:21:50 -0400
> Organization: America Online, Inc. (1-800-827-6364)
> Lines: 7
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> Reply-To: brucerat at aol.com (BruceRat)
> NNTP-Posting-Host: newsbf02.mail.aol.com
> Using a simple photometer to detect digestion of iodine-stained
> starch by salivary amylase, I have gotten a graph that is FAIRLY
> modeled by the Michaelis-Menten equation. But it deviates
> Do you have any suggestions as to why?
> Bruce Ratcliffe, AP Bio teacher, Edison High School Fresno, CA
> BruceRat at AOL.COM
If the iodine is added too early or too strongly it will interfere with
the enzymic activity. The same mess-up happens with most iodometric
titrations if the iodine is added too soon.
Univerisity of Reading, UK.
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