Forday Wayne Lee
fwl at titan.np.ac.sg
Sun Nov 5 19:32:01 EST 1995
> Using a simple photometer to detect digestion of iodine-stained
> starch by salivary amylase, I have gotten a graph that is FAIRLY
> modeled by the Michaelis-Menten equation. But it deviates
> Do you have any suggestions as to why?
The problem could be that you are using a colloid substrate. The kinetics
that you are measuring could be those of mass transfer and not of the
Try doing the experiment at different stirring/shaking speeds and if Vmax
increases with stirring speed, then you are mass transfer limited.
Wayne Lee Forday, Biotechnology Department
Ngee Ann Polytechnic, Singapore
fwl at np.ac.sg
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