Transformation of Ps. aeruginosa/stutzeri

Vincent Mulholland mulholl at sasa.gov.uk
Mon Oct 23 04:40:44 EST 1995


In article <1995Oct9.181237 at ania.path.ox.ac.uk>, sbaker at molbiol.ox.ac.uk (Simon Baker) says:
>
>I am looking for a method to transform Pseudomonas species.
>I'm aware of the electroporation methods, but if there are
>any "calcium-chloride-like" protocols, I'd be very interested
>to hear of them

Simon,

Have a look at Steward & Carlson (Annu. Rev. Microbiol. 40:211 (1986)). 
I don't have the article to hand at the moment, but I think it might
help.


Vince Mulholland
Scottish Agricultural Science Agency.




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