species specific bacterial probes(DNA, Monoclonal antibody)

u2528082 at ucsvc.ucs.unimelb.edu.au u2528082 at ucsvc.ucs.unimelb.edu.au
Fri Sep 1 00:09:46 EST 1995


In article <41ndui$he6 at ixnews2.ix.netcom.com>, chicago at ix.netcom.com (Chicago) writes:
> In <mbransde-080895165122 at mpg7-97.appbio.utas.edu.au>
> mbransde at postoffice.newnham.utas.edu.au (Matthew Bransden) writes: 
>>
 For universal PCR primers or species specific primers, the 16S rRNA gene
is obviously  the best characterised and most likely candidate.
For universal, compare sequences of widely unrelated bugs, on say Genbank.
Species specific 16S- rRNA sequences should be under orgs. name. Design your
own primers.
In my experience with mycoplasma, this approach works well. Choose a few primer
pairs to find the most efficient combination(s).

Yours,
John



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