differences between bacteria (2)

Eberhard Kniehl 100674.1442 at CompuServe.COM
Mon Sep 18 15:42:43 EST 1995


 In contrast to EC and SL, SH is non-motile (means has no 
flagellae); EC can metabolize lactose (lactose+, yellow with acid 
indicator) in most cases, SL and SH cannot (lactose-). SL can 
reduce sodium-sulfite (H2S+, black with bismut-citrate 
indicator).
ER is also considered to be an "enterobacteriacea", but is a 
plant pathogen; AZ is a soil germ.
VC is a water-borne germ as well, shedded through intestinal 
tract of e.g. mammals and may reside in e.g. sea-food; it is - in 
contrast to all others in your list - no (straight) rod, but a 
comma-like bacteria.
LP is a water-borne germ, which survives best intracellularly (in 
amoeba (water) or human macrophages (lung)), also- in contrast to 
all others - at higher temperatures (40 - 50 C).
HI is an organism, which requires hemin from erythrocytes for 
growth; it therefore will never survive in the environment; it is 
- besides LP - the only microaerophilic (means requires CO2 
enriched culture) in your list, all other are aerobs (it looks 
strange in your list of possible environmental isolates).
For isolation of mentioned bacteria, you may need to pretreat the 
specimens (enrichment or selective media). SL,EC,SH,HI grow best 
at 36-37C, at pH 7.2; HI needs hemin and CO2.
LP needs  charcoal, iron- and cystein-supplemented agar (e.g. 
BCYE-agar) at pH6.9. VC is best cultivated in an alcaline peptone 
bouillon (pH 8.5). Good luck 

-- 
Eberhard Kniehl
Karlsruhe - Germany



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