non-radioactive marker enzyme assays

Torsten Boerchers borcher at uni-muenster.de
Sat Sep 23 12:46:39 EST 1995


Is anybody aware of a nonradioactive method
to determine the enzymes that confer antibiotic
resistance, in particular vs.kanamycin or hygromycin?
Those enzymes are  phosphotransferases (nptII, hpt) and 
inactivate the antibiotics by phosphorylation.I am looking
for an alternative to the thin layer chromatography
with subsequent autoradiography.

Furthermore, does anybody know to which amount these
enzymes are actually expressed in the transgenic plants?
Can they be seen in Western Blotting and are antibodies
available?

Any hints in the right direction are highly appreciated.
Torsten
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                          Torsten Boerchers                            /
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