medium for water bacteria

K N and P J Harris ecoli at cix.compulink.co.uk
Thu Apr 4 16:27:01 EST 1996


> ==========
> bionet/microbiology #2577, from kisand at kadri.ut.ee, 740 chars, 3 Apr 
1996 19:53:29 
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> Article: 3528 of bionet.microbiology
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> From: kisand at kadri.ut.ee (Veljo Kisand)
> Newsgroups: bionet.microbiology
> Subject: medium for water bacteria
> Date: 3 Apr 1996 19:53:29 GMT
> Organization: Tartu University, Estonia
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> Hi!
> 
> I would like to grow up as many as possible colonies from eutrophic 
lake
> water. Which medium should I use?
> 
> thanx in advance
> 
> Veljo Kisand
Eutrophic lake water,
is still pretty thin stuff, so - don't use any medium that is too rich. 
Avoid Nutrient Agar and similar medical type concoctions. My first 
choice would be to get some tryptic soy broth and try it at a few 
different concentrations (e.g. 1%, 0.3% 0.1 % w/v) and solidify the 
medium with the best quality pure agar (or agarose) you can afford.
Works well for soils,
Peter Harris,
Reading Univ, UK.
AKA P.J.Harris at reading.ac.uk




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