pemanuel at umabnet.ab.edu
Tue Apr 9 08:41:21 EST 1996
My electroporation of bacterial cells didn't work.
I used the BIO RAD E coli gene pulser which lacks the ability to alter
Their self test indicates the machine is OK.
The cells are XL-Blue F`(300ul) at 2.5kV in a 0.2cm gap cuvette (Cold)
The plates are 100ug/ml carbenecillin.
No colonies. nothing with my ligation mixes. I thought too high in salt.
Then I tried Supercoiled puC18. No colonies when I added 5 ul of a
.01ng/ul solution of pUc18 which came from Stratagene.
I even bought some Stratagene electro comp cells and they didn't work either.
Why isn't my electroporation working?
Peter Emanuel Ph.D.
University of Maryland
School of Medicine
More information about the Microbio