Isolation of spores

[Dennis Gaunt]

Doug Stemke (dstemke at umabnet.ab.umd.edu) wrote:
: On Wed, 24 Jul 1996, T. Reid wrote:
: 
: > Hi.  I have already posted this to the mycology group but thought I might hit a bigger 
: > audience within this group.  Does anyone know of a reasonable method to isolate intact 
: > fungal spores from soil samples?   I am looking for a non-culture method; filtration,
: > centrifugation, etc.  I am specifically interested in Histoplasma
: > capsulatum.
: > 
: > Thanks,
: > Tom Reid
: > Cincinnati, Ohio
: > 
: > 
: Nasty organism!  For Streptomyces we occasionally would pass orgaisms
: through sterile cotton in a syringe.  It worked rather nicely to separate
: spores from vegatative cells.  I suppose it *might* work with true fungi.
: 
: Hope this helps.
: 
: Douglas Stemke
: Biological Process Technology Institute
: University of Minnesota
: 



A good reference, if you can find it, is Smith and Furcolow, The Journal
of Laboratory and Clinical Medicine, 1964, 64:342-348.  Very briefly,
mineral oil is used to float Histoplama conidia out of a sample.
-- 
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| Dennis D. Gaunt                   | Internet: dgaunt at uhl.uiowa.edu | 
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