PCR cloning-LIC

Bradley Turner bsturner at MBCRR.HARVARD.EDU
Thu Aug 1 00:41:10 EST 1996


Dear Nitin Baliga,

You might want to consider LIC (ligation-independent-cloning),which
is described in the references below, as allowing directional cloning
of PCR products without using ligations, restriction enzymes or T/A
vectors.

Rapid, reliable ligation-independent cloning of PCR products using
modified plasmid vectors
RS Haun, IM Serventi, J Moss
Biotechniques 13(4)515-8, 1992

Ligation-independent cloning of PCR products (LIC-PCR)
C Aslanidis, P deJong
Nucleic Acids Research 18(20)6069-74, 1990

Novel methods for cloning and engineering genes using the polymerase
chain reaction
A Rashtchian
Current Opinion in Biotechnology 6:30-36, 1995

Ligation-independent cloning of PCR products with primers containing
nonbase residues
S Kaluz, APF Flint
Nucleic Acids Research 22(22)4845, 1994

Also, Pharmingen (800-848-6227) and Novagen (800-526-7319) both sell
LIC vectors and kits [no affiliation with these companies].
I hope you find this helpful,
Brad Turner

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		    Bradley Turner
	         Beth Israel Hospital

Harvard Medical School		617-667-1215 phone
Division of Gastroenterology	617-667-2767 fax
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