UV mutagenesis

Steven Goldberg goldberg at bms.com
Mon Aug 5 13:54:42 EST 1996


I am preparing to do some UV mutagenesis of E. coli but can't find a
sufficiently detailed protocol.  The only thing I can recall is that you
have to suspend the cells in a non-UV absorbing buffer like PBS.  But I
can't remember how long (or, how many Joules one uses) to treat the cells
and if you have to let them recover before plating them out.  If anyone
has some experience with this procedure I would appreciate hearing from
you.

Thanks.

Steve



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