Vibrio fischerii

Brent Gilbert bg005d at uhura.cc.rochester.edu
Thu Aug 8 10:24:42 EST 1996


In <4ucnal$iof at news.sct.fr> pjurgens at worldnet.fr (Pete Jurgens) writes:

>I am trying to get Vibrio fischerii to emit light and, despite 
>respecting the recommendations of Farghaly concerning media and 
>temperature cannot find any light even at the most sensitive settings 
>of a PMT. Has anyone worked with this organism with success? I would be 
>most grateful for any ideas.

The LuxR gene is activated only at very high levels of inducer. It is
imperative that you have as many organisms as possible in a confined area.
What sort of media are you growing on? The problem many be with the culture.
-- 
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Brent Gilbert (bg005d at uhura.cc.rochester.edu)
"If you choose not to decide, you still have made a choice" -Neil Peart



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