How do I grow Alcaligenes without flocs?

John Kurt Jackson Jacks052 at MHUB0.TC.UMN.EDU
Mon Aug 12 22:23:48 EST 1996


I've tried rich media and minimal media, but Alcaligenes eutrophus 
continues to flocculate under my watchful gaze.  This makes OD readings 
impossible.  Is there something I can add to the media, or some ion 
concentration I should change to obtain a homogeneous culture?  I'm sure 
this must be a common problem in bioreactors, but I can't find anything 
in the literature...






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