galperin at biotek.mcb.uconn.edu
Mon Aug 12 14:01:08 EST 1996
bg005d at uhura.cc.rochester.edu (Brent Gilbert) writes:
pjurgens at worldnet.fr (Pete Jurgens) writes:
>>I am trying to get Vibrio fischerii to emit light and, despite
>>respecting the recommendations of Farghaly concerning media and
>>temperature cannot find any light even at the most sensitive settings
>>of a PMT. Has anyone worked with this organism with success? I would be
>>most grateful for any ideas.
>The LuxR gene is activated only at very high levels of inducer. It is
>imperative that you have as many organisms as possible in a confined area.
>What sort of media are you growing on? The problem many be with the culture.
It also helps to get really good aeration and to add about
1 mM benzoic acid (don't ask me why!).
Michael Galperin galperin at biotek.mcb.uconn.edu
More information about the Microbio