Particle Filtration Problem

John Bates ae55 at cityscape.co.uk
Fri Dec 6 14:43:38 EST 1996


Can anyone suggest an inert membrane for the filtration of plasma samples 
containing variable levels of protein precipitate (eg denatured protein) 
and/or microorganisms.

I am having problems reproducibly filtering these samples using 0.45 
polypropylene filter. Some are OK but others block. It is a particular 
problem if the plasma is buffered at high or low pH .. which causes
precipitation. I would prefer to avoid a centrifugation step. 

Any suggestions would be gratefully received.

John Bates






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