lysis of Ecoli

[Robert Presswood]

J. Dennis Pollack wrote:
> 
> Help! I need a lysis technique to release cytoplasmic enzymes from transformed
> Ecoli. I cant denature the proteins and hence cant use some of the more
> frequently advertised techs. I suspect lysozyme will work, but I dont want to
> sonicate if I can avoid it. The procedures in Maniatis or Current Protocols
> are not detailed enough. Can anyone help? Thanks a lot. Dennis

Try a quaternary ammonium detergent(say 0.1% or so) in an appropriate
buffer.