None toxic buffer and dye for electrophores

Vu Nguyen Thanh thanh at
Tue Feb 6 18:31:16 EST 1996

Hi there,
I need to determine the charge (positive/negative/none) of unknown 
growth factor in a mixture for further purification.  I am going to do 
electrophores and then cut the gel for testing.  I guess it has a polyol 
part (consists some saccharide units?).  I don't know which kind of buffer 
and dye should I use for this.  I would appreciate it very much if anyone
could give me an advice.

Vu Nguyen Thanh
Dept.  Microbiology & Biochemistry
The University of Orange Free State
Box 339,  Bloemfontein 9300, South Africa
e-mail: thanh at  Fax: 27-51-4482004

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