EPS serapation from bacteria

Brian Hoyle brianh at gov.nb.ca
Tue Feb 20 08:07:19 EST 1996

Exopolysaccharide can be purified using the procedure of Doig, 
P., et al., Infection & Immunity 55: 1517-1522 (1987). I have 
used this procedure to purify the EPS from planktonic P. 
aeruginosa. It should be applicable to biofilms, but I think 
that you would need a lot of starting material. Preparing 
biofilms "in bulk" is hard work. Good luck.

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