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[Michael Kolotila x3887]

Dear Matt;
I have had that experience and what I was able to atrribute it to or at 
least when I got new ammonium persulfate the problem disappeared.  
Therefore, my first response is to replace the ammonium persulfate by 
buying a new bottle and getting some from another lab unless their gels 
are setting.
Good luck.
Michael

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 Michael P. Kolotila, Ph.D.        * e-mail: mkolotila at necc.mass.edu   
                                   *
 Biotechnology Program Coordinator *          
 Department of Natural Science     * voice :  508-374-3887
 Northern Essex Community College  *
 100 Elliott Way                   * fax   :  508-374-3723
 Haverhill, MA  01830-2399         *        
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On Wed, 28 Feb 1996, M.C. Bean wrote:

> Is anyone else out there, familiar with the experience of pouring a 
> polyacrylamide gel really carefully, ticking off every step as you do it and 
> it not setting?
> 
> Matt
> 
>