Gram-Specific DNA probes

chris chris.michiels at agr.kuleuven.ac.be
Tue Jan 9 08:00:35 EST 1996


I doubt that it is possible to make a DNA probe specific for 
gram-negatives. Indeed, the phylogenetic tree of all bacteria based on 
ribosomal RNA sequence homology shows that the gram-positive branch is 
embedded in gramnegative branches. A probe specific for the gram-positive 
branch may be possible, although this branch contains some bacterial 
groups that stain gram-negative: the Mycoplasmas (wall-less bacteria) and 
Fusobacteria.(ref: Olsen et al. 1994, J. Bacteriol. 176, 1-6).

Chris Michiels
K.U.Leuven
Lab Food Microbiology
K. Mercierlaan 92
B-3001 Heverlee
Belgium
Tel: +32 - 16 321578
Fax: +32 - 16 321997




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