Gram-Specific DNA probes

chris chris.michiels at
Tue Jan 9 08:00:35 EST 1996

I doubt that it is possible to make a DNA probe specific for 
gram-negatives. Indeed, the phylogenetic tree of all bacteria based on 
ribosomal RNA sequence homology shows that the gram-positive branch is 
embedded in gramnegative branches. A probe specific for the gram-positive 
branch may be possible, although this branch contains some bacterial 
groups that stain gram-negative: the Mycoplasmas (wall-less bacteria) and 
Fusobacteria.(ref: Olsen et al. 1994, J. Bacteriol. 176, 1-6).

Chris Michiels
Lab Food Microbiology
K. Mercierlaan 92
B-3001 Heverlee
Tel: +32 - 16 321578
Fax: +32 - 16 321997

More information about the Microbio mailing list