pGEX system

meller yael meller at ccsg.tau.ac.il
Sun Jan 14 13:33:24 EST 1996


Hi,
I have the plasmid pGEX-2T containing the gene insert. These gene doesn't 
have his own sequence for transcription termination, but can be expressed 
in the plasmid in frame with Sj gene (on Sj's 3'-terminus). I would like 
to reclone my gene together with Sj's transcription terminator from pGEX 
to another plasmid.
So, any information about transcription termination for genes cloned in 
pGEX or for Sj gene would be very appreciated!
Thanks in advance. Il.
f139994 at ccs.tau.ac.il




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