Clostridium difficile

Tracey Ann Ristow Lozano tar205 at
Thu Jan 18 14:02:06 EST 1996

We are trying to isolate genomic DNA from Clostridium difficile isolates 
and are having trouble lysing them.  We are treating with Lysozyme (50 
mg/ml) for 30" and our lysing agent is Guanidium-EDTA-Sarkosyl.   Anyone 
have any ideas?

Tracey Ann Ristow Lozano
Northwestern University, Evanston, IL.   USA
tar205 at

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