How do you introduce plasmid DNA into B. megaterium?

David A. Mullin damullin at MAILHOST.TCS.TULANE.EDU
Fri Jan 26 00:08:12 EST 1996


        I plan to construct a gene replacement in Bacillus megaterium.  Can
someone who knows about B. megaterium suggest the best way to introduce a
suicide plasmid? (eg, electroporation, transformation [does B.m. develope
competence naturally?], or conjugation).

        Sincerely,


        D. Mullin




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