Agrobacterium OD readings


Fri Jan 26 01:23:46 EST 1996



In terms of growing agrobacterium, most of the articles I have read,
people grow agro to OD600 = 0.5-0.8 which is supposed to be
log phase.  In terms of a conversion factor I have seen

OD600 of 1.0 = 5 X10^8 cells/ml

I have also seen others that are similar but about 10 - 100 fold
different.  Does anybody have any info on growth of agrobacterium, and
if possible, the best way to generate a 2L volume that contains about
5X10^8 cells/ ml? 


Jason
jjgo at gnv.ifas.ufl.edu





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