Anaerobic Medium?

rand777 at ids.net rand777 at ids.net
Tue Jun 25 07:07:06 EST 1996


In Article <31D07789.6ADF at mbox.fam.admin.ch>
Michael Casey <Michael.Casey at mbox.fam.admin.ch> writes:
>I am studying the rate of growth of Clostridium tyrobutyricum in acid medium
>and have found them to be very sensitive to the presence of oxygen. I follow
>the growth rate by measuring the turbidity at 650 nm. I have tried using
>different techniques to reduce the oxygen content of the medium but with 
>little success. 
>
>Gassing with CO2 does not reduce the oxygen content sufficiently.
>
>The addition of cysteine to the medium is also not sufficient but the
>presence of sodium thiogllycolate inhibits growth. 
>
>The addition of Oxyrase, a commercial enzyme preparation which removes 
>oxygen, causes turbidity and prevents accurate measurement of growth.
>
>Does anyone have an idea how I could reduce the oxygen content of
>the medium without causing turbidity?
>
>Thank you.

Please take a look at our webpage for anaerobic PRAS media:
          http://www2.ids.net/~rand777/home.htm
R.Randolph

s
>-- 
>Michael Casey
>Swiss Federal Dairy Research Institute
>3097 Liebefeld
>Switzerland
>Tel: (+41) 31 323 81 79
>FAX: (+41) 31 323 82 27
>email Michael.Casey at fam.admin.ch



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