K N and P J Harris
ecoli at cix.compulink.co.uk
Fri Mar 1 15:38:11 EST 1996
> bionet/microbiology #2156, from jlaveck at cpcug.org, 1272 chars, Thu 29
Feb 1996 01:16:22
> Article: 3103 of bionet.microbiology
> From: jlaveck at cpcug.org (Jerry LaVeck)
> Newsgroups: bionet.microbiology
> Subject: Re: PAGE
> Date: Thu, 29 Feb 1996 01:16:22 GMT
> Organization: Express Access Online Communications, USA
> Lines: 17
> Message-ID: <3134fccf.973790 at news.digex.net>
> References: <4h1p8j$8bs_001 at leeds.ac.uk>
> NNTP-Posting-Host: cpcug.org
> X-Newsreader: Forte Agent .99d/32.182
> On Wed, 28 Feb 1996 14:35:21 +0000 (GMT), MICMCB at leeds.ac.uk (M.C.
> Bean) wrote:
> >Is anyone else out there, familiar with the experience of pouring a
> >polyacrylamide gel really carefully, ticking off every step as you do
> >it not setting?
> I haven't poured a gel in a loooong time, but I remember having your
> experience a few times. One cause is oxygen - it inhibits
> polymerization. You can take care of it by de-gassing the acrylamide
> mixture. Watch out here - you end up with something that sets up like
> a rock before you get it poured! The other cause is the catalyst -
> ammonium persulfate can go bad quickly. Make up a fresh mixture and
> use it in your next gel.
> Good luck!
Good idea, that's what I call useful information,
Just about to get involved.
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