another question about SDS-PAGE

Michael Kolotila x3887 mkolotila at necc.mass.edu
Mon Mar 4 10:24:35 EST 1996


Dear Erma;
Two things that you can do; use larger combs so that the well will be 
larger even with shrinkage or you can continue to add stacking gel for a 
while to increase the size of you wells as the stacking gel polymerizes 
and shrinks.
Good luck.
Michael

******************************************************************************
 Michael P. Kolotila, Ph.D.        * e-mail: mkolotila at necc.mass.edu   
                                   *
 Biotechnology Program Coordinator *          
 Department of Natural Science     * voice :  508-374-3887
 Northern Essex Community College  *
 100 Elliott Way                   * fax   :  508-374-3723
 Haverhill, MA  01830-2399         *        
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On 1 Mar 1996, Erma Lai wrote:

> I have a problem when I prepare the stacking gel. I found my separating
> gel polymerized very well but the stacking gel didn't. The problem is that
> the wells of the stacking gels were shrinked after polymerized. Therefore,
> I only can load small amounts of the samples. Hope someone will help me!
> 
> Erma
> 
> 



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