another question about SDS-PAGE

Baiqing at jircas-os.affrc.go.jp Baiqing at jircas-os.affrc.go.jp
Thu Mar 14 16:21:37 EST 1996


In article <emlai-0103960121580001 at reqf-027.ucdavis.edu>, <emlai at ucdavis.edu> writes:
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> From: emlai at ucdavis.edu (Erma Lai)
> Newsgroups: bionet.microbiology
> Subject: another question about SDS-PAGE
> Date: 1 Mar 1996 09:18:49 GMT
> Organization: U. C. Davis
> Lines: 6
> Message-ID: <emlai-0103960121580001 at reqf-027.ucdavis.edu>
> NNTP-Posting-Host: reqf-027.ucdavis.edu
> 
> I have a problem when I prepare the stacking gel. I found my separating
> gel polymerized very well but the stacking gel didn't. The problem is that
> the wells of the stacking gels were shrinked after polymerized. Therefore,
> I only can load small amounts of the samples. Hope someone will help me!
> 
> Erma
> How is your stacking gel concentration, in percentage? try to photopolymerize it around 30C degree (Temperature).





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