Scanning Electron Microscope

[Robert McGehee]

Hello, I am working on research project using A. tumefaciens and needed 
to take a scanning electron micrograph of them and have had some 
problems.  Initially, cells were removed from agar plates and dried on 
copper before being gold-coated.  Upon viewing, the microscope could not 
resolve the different cells, only a large mass.  Is there a special 
method for viewing bacterial cells under a SEM?  I will try drying cells 
at a significantly less concenetration however.  Any suggestions?

Robert

respond:	rmcgehee at teclink.net