Haishan Xiong wrote:
>> I have been experiencing some problems with growing E. coli in M9
> minimum media. I tried to grow JM109 and XL1-Blue cells in M9 +
> glucose. Neither grew. I did not find any difficiency in terms of
> their genotype. How can I get around with this problem?
> I appreciate your kindly help.
How good is your water? House distilled water can have many organic
contaminants which may inhibit bacteria growth. Also if you use a
millipore system be sure to change the filters fairly frequently. This
has been a problem in a lab I have worked in before.