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Minimal media

Joseph E. Mayer Jr. mayerje at sluvca.slu.edu
Sun Nov 3 23:57:03 EST 1996


Haishan Xiong wrote:
> 
> I have been experiencing some problems with growing E. coli in M9
> minimum media.  I tried to grow JM109 and XL1-Blue cells in M9 +
> glucose.  Neither grew.  I did not find any difficiency in terms of
> their genotype.  How can I get around with this problem?
> I appreciate your kindly help.
> 
> Haishan
How good is your water? House distilled water can have many organic
contaminants which may inhibit bacteria growth. Also if you use a
millipore system be sure to change the filters fairly frequently. This
has been a problem in a lab I have worked in before.



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