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tears

Michael Kolotila x3887 mkolotila at necc.mass.edu
Fri Nov 8 09:38:43 EST 1996


Hi Richard;
  You are right.  I was working with M. luteus in my lab and that name
stuck.  Sorry.
   Michael

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 Michael P. Kolotila, Ph.D.        * e-mail: mkolotila at necc.mass.edu   
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On 7 Nov 1996, richard wrote:

> In article <Pine.D-G.3.93.961106153410.4171A-100000 at necc.mass.edu>, mkolotila at necc.mass.edu (Michael Kolotila x3887) says:
> >
> >Dear Jane;
> >  I assume that you mean lysozme and lysosomal.  Micrococcus luteus is the
> >organism used with lysozme.  Dried Micrococcus luteus can be bought from
> >Sigma Chemical Co.  Good luck.
> >  Michael
> >
> >
> I don't have ready access to the latest Bergey's and perhaps 
> M. luteus is nowadays the assay organism for lysozyme.  In my day,
> ancient history circa 1967, M. lysodeikticus was the assay organism
> for lysozyme.  Has this been sorted out in a systematic way or is
> luteus being used because it is more readily available and more familiar?
> 
> In any case the cell wall stucture of micrococci make them suitable for
> lysozyme assays.  If I remember correctly, and perhaps I don't, the
> cell wall is pure peptidoglycan free of cross linking gunk like teichoic
> acids.  Am I correct? 
> 
> 




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