Now I am doing a project about petroleum utilizing bacteria. I need to isolate petroleum
utilizing bacteria from sea. However, I still cannot get any bacteria culture from sea. Here is
the medium I used and the general environmental conditions of Hong KOng.
Substrate: Diesel from Oil station (brand name: Mobil)
or Motor oil from factory
Medium ; K2HPO4 ,KH2PO4, MgSO4, NaCll, CaCl , NH4NO3 in deioonised water ewith trace e lement t
stock (H3BO3 , ZnSO4.7H2O , CuSo4.5H2O , MnSO4.H2O ,FeCl3.6H2O , (NH4)6Mo7O24.4H2O )
this component is taken from Can. J. Micorbiol. Vol. 20 pp955-862.
Environmental conditions:Temperature of seawater 25 C - 27C
Dissolved oxygen in water Site 1 : 3.3 mg/L ( the Lowest one ))
Site 2 : 6.73 mg /L
Turbidity 6.59 NTU to 12 NTU
Salinity around 32ppt
Incubating Temperature : 22C (room temperature)
I had incubated the sample water with above medium and Diesel for a month , but no bacteria
grew on it. Now , I used motor oil as substrate for a weeks and also seems no growth .
Up to now, I don't Know what the problem is. Would you please help me to solve the problem or
give me more information if you think that is helpful.
Thanks for your attention !