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Q: No antibiotic resistance gen?

Frank Maiwald maiwald at mpiz-koeln.mpg.de
Fri Nov 8 04:16:19 EST 1996


Andre Habel wrote:
> 
> Hy,
> 
> I would like to construct a plasmid,which has no antibiotic resistance
> gen. Instead I would like to integrate something else (DNA-sequence,
> small gene, etc.) for convinient selection of positive clones. Due to the
> fact that I want to keep my plasmid as small as possible, the
> selection-marker / sequence should also be as small as possible.
> I would appreciate any ideas/suggestions on that particiular case.
> Plaese e-mail your ideas to ahabel at pasteur.fr
> 
> Thanks in advance :-)
> 
> Andre


What abou the blue/white selection using the lacZ gene
(alpha-complementation) ? You should take care to choose a very stable
replicon e.g. one with the pVS1 origin of replication.

Good luck					Frank



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