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Looking for Fluorimetric assays for micrbial metabolism

Userid USERID at mops.wl.com
Tue Nov 19 13:48:44 EST 1996


Does anyone have a good assay (or reference) to fluorimetric assats of 
glycolytic activity?  I have used MUG and other methylumbelliferyl 
compounds, but am not sure that a substrate exists for glucose-specific 
enzyme systems.  Would 4-MU-glucoside work?  I want to evaluate effect of 
various membrane-active compounds on maicobial metabolism.



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