Help on growing SRB on agar plates

Joseph E. Mayer Jr mayer at sluvca.slu.edu
Thu Nov 28 00:45:31 EST 1996


In message <Pine.GSO.3.95.961123111749.15450A-100000 at design.eng.uci.edu> -
Arul Jayaraman <arul at design.eng.uci.edu>Sat, 23 Nov 1996 11:22:25 -0800
writes:
:>
:>Hi
:>I am working with sulfate-reducing bacteria (Desulfovibrio vulgaris and D.
:>gigas). I am having difficulty in growing these bacteria on agar plates/
:>solid medium. I have tried growing them on Modified baar's medium agar
:>plates (the medium that ATCC prescribes for growing these bacteria in
:>liquid cultrues) and on Iverson Medium plates (Trypticase soy agar,
:>magnesium sulfate and sodium lactate). I incubate the plates in a gasPak
:>chamber (hydrogen + carbondioxide environment). But growth is very poor. I
:>would
:>appreciate it if anyone can give me suggestions/pointers on growing SRB on
:>plates.
:>
:>Thank you
:>Arul
:>
:>---------------------------------------------------------------
:>Arul Jayaraman
:>Dept. of Chemical & Biochemical Engineering
:>University of California at Irvine
:>Irvine, CA 92697-2575
:>Ph : (714)-509-1060 (H)
:>     (714)-824-8389 (L)
:>http://www.eng.uci.edu/~arul/
:>
:>Try washing the agar several times in cold water. To this just add agar to a large breaker, add an aliqout of cold water and stir. After a few minutes filter the agar and repeat the process 6 times or so. After the last filtration step, I usually put the agar on some paper towels and let the agar dry. Store excess agar for later use. This removes organic matter that inhibits many lithotrophs. Hope this helps.

Joe
:>
:>







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