Help on growing SRB on agar plates

K N and P J Harris ecoli at cix.compulink.co.uk
Sat Nov 30 14:42:03 EST 1996


Hello Arul.
Unfortunately the vast majority of agars contain carbohydrates that can 
be used by all and sundry. This might be just enough to mess up what you 
are trying to do.
If you doubt it, try plating out a soil suspension (or your favourite 
inoculum) on water agar, and when a few colonies appear flood the plate 
with iodine. No starch was added but you will find that there are areas 
of clearing which means that SOMETHING that was iodine reactive was 
removed. OK ?
Best wishes,
Peter Harris,
Reading.UK.
AKA <P.J.Harris at reading.ac.uk>




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