Sequencing 7-8 kb deletion
Sharon Jean Kelley
skelle2 at unix1.sncc.lsu.edu
Wed Oct 9 11:42:05 EST 1996
I wish to sequence a large deletion to determine its break points. I
know from restriction analysis that a 13 kb region of lambda DNA has been
reduced to 5 kb. Any advice on how to tackle this problem would be
appreciated. I have experience with cycle sequencing using S35 labeled
dATP. I do not have access to an automated sequencer.
Thanks in advance for any advice.
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