Sequencing 7-8 kb deletion

[Sharon Jean Kelley]

Hi!

I wish to sequence a large deletion to determine its break points.  I 
know from restriction analysis that a 13 kb region of lambda DNA has been 
reduced to 5 kb.  Any advice on how to tackle this problem would be 
appreciated.  I have experience with cycle sequencing using S35 labeled 
dATP.  I do not have access to an automated sequencer.

Thanks in advance for any advice.

Sharon