AGROBACTERIUM/YEB

Frank Maiwald maiwald at mpiz-koeln.mpg.de
Fri Oct 18 09:30:05 EST 1996


Dear reader,

I am doing a plant transformation project using Agrobacterium
tumefaciens. One of my strains - LBA4404(pTOK233) [Hiei et al. Plant
J.6:271-282, 1994]- will not grow on YEB-medium with the proper
selection. Even without any selection it does not grow on this medium
whereas it is quite happy on minimal media like AB. LBA4404 without a
binary plasmid or with other plasmids grows on YEB like every well
mannered Agrobacterium.

Can anybody suggest reasons for this phenomenon just to cure my
curiosity?

Please post in this newsgroup or email to maiwald at mpiz-koeln.mpg.de

				Thanks in advance!



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