Question : Cultivation of fungy
yersinia at GATE.CYBERNEX.NET
Sat Oct 26 10:23:26 EST 1996
Steve Teasdale writes:
<I am producing penicillium spores on solid media. It is space demanding.
Does someone know about any cultivation method (liquid or semi-liquid)
that could help me produce latger amount of spore from my fungy.>
I don't know of a liquid method of cultivation for any mold, but your
post made me curious. When you say you're producing on solid media, how
are you doing it (plates, slants, roux bottles)? And more importantly,
how much spore do you need? We make spore suspensions of A. niger for
preservative efficacy and growth promotion testing. The procedure I use
involves inoculating six slants (Sabouraud Dextrose Agar). When the
inocula on the slants sporulates, I wash off the spores with Saline/Tween
80. The washings are inoculated on the surface of five R2A roux bottles.
The roux bottles, of course, have much greater surface area than slants,
so you get a greater amount of spores. The harvest from the roux bottles
is centrifuged. The decanted supernatant contains enough spores (10 to
the 4) for growth promotion media testing, whereas the reconstituted
pellet yields at least 10 to the 7, the amount we need for preservative
efficacy. These suspensions - kept refrigerated in screw-cap test tubes
with glass beads - are good for a year.
The roux bottles don't take up that much space, so I opine. But then
again, I don't know how big your incubator(s) is(are) either.
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