Purified mRNA

Umnarj Paeratakul umnarj at mozart.inet.co.th
Thu Oct 31 20:27:26 EST 1996


mcamargo at usp.br (Maria Evangelina de Camargo) wrote:
>
>	Hi, 
>I need a good protocol to preper RNAm. If you have, please, help me!
>	
>	Maria.
>

Dear Maria,

Need more information!  What is your source?  Mammalian cells?
What will this used for?

I do my RNA extraction by using Guanidine isothiocyanate / ultra
centrifugation method (paper by Chomzinski and Sacchi - if I 
spell it right).  After that I use oligo dT cellulose to 
pull out poly A+ RNA from the total RNA.  Extracting RNA is 
a pain in the neck, you have to be very careful not to 
contaminate it with ribonuclease.  Otherwise all RNA will be
degraded.


You might want to check a book called Molecular Cloning published
by Cold Spring Harbor Laboratory.  This is a very good book.

Good Luck

Umnarj Paeratakul
Thailand




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