Isolation protocol for inclusion bodies

Niek Schroten N.Schroten at med.ruu.nl
Sun Sep 15 14:15:40 EST 1996


Hi,

I'm trying to isolate a 103 kD protein in BL21(DE3) in inclusion
bodies. Is anyone familiair with isolation of inclusion bodies in
combination with a protein of this weight ? Does anyone have a useful
protocol for inclusionbody isolation ?
Thanking you in advance for your help,

Niek Schroten.




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